Center for Microbiology and Phage Therapy
Field of Interest
The center focuses on the potential of using phages and their new biotechnology products such as Endolysin in the treatment of bacterial infections in food, veterinary and human. We are aiming to develop new state-of-the-art products involving phages and to apply different viable forms of phages such as powders, sprays, wash and develop a new delivery system to combat antibiotic resistant bacteria and to treat challenging diseases thus improving human and animal health.
The major lab techniques or research facilities that are unique to the center research area
Phage isolation, amplification, and typing techniques: for the production of phage for phage therapy application (biocontrol) to control bacterial contamination of food to improve food safety. Molecular techniques: for the production of phage based products to control bacterial contamination. State-of-art delivery techniques: Develop effective natural, reliable and cost effective products that can be used in innovative delivery systems to control bacterial infections. VITEK MS analyzer: by applying the internal and Original Equipment Manufactural (OEM) Methods, automated VITEK MS analyzer will be used for phenotyping characterization for fast and accurate detection of different pathogenic microorganisms. Freeze Dryer: For long term preservation of different reference strains at optimum and suitable cryoprotectant agents in accordance to ISO standards. Fermenter: For Large scale production of phage and for phage products productions.
The Center PI
Prof. Dr: Ayman El-Shibiny
Professor of biomedical sciences
Director of Center for Microbiology and Phage Therapy
Director General for Research
Brief about the PI
Dr. Ayman El-Shibiny is currently working as a professor of biomedical sciences, director general for research and vice director of accreditation unit at the University of Science and Technology at Zewail City. El-Shibiny got his Doctor of Philosophy degree in Microbiology from The University of Nottingham, UK in 2006. Prior to joining Zewail City, El-Shibiny worked as a postdoctoral fellow at a number of universities including University of Nottingham (UK), Cardiff University (UK), and The Evergreen State College (USA). As a microbiologist, El-Shibiny’s research interests cover a broad range of topics related to Food Safety and Human Health. He established the first Phage Therapy lab in Egypt at Zewail City. His main research area is Phage Therapy and his current research interests include the therapeutic use of bacteriophages as a possible alternative to treat and control antibiotic-resistant pathogens in food, veterinary, and human.
Contact e-mail of the Director
A list of the researchers in the center and their affiliation to the lab (technicians, lab managers, postdocs, Ph.D. students, masters students, research assistants, interns, or junior researchers)
1. Mr. Mohamed Gad, Lab Manager
2. Abdallah AbdelSattar, research assistant and MSc student
3. Mennatallah Mamdouh, MSc student
4. Nada Adel, research assistant, and MSc student
5. Fatma Abdelrahman, research assistant, and MSc student
6. Anan Safwat, research assistant and MSc student
7. Rana Ramadan, research assistant, and MSc student
8. Samar Hamed, MSc student
9. Nedaa Mohamed, MSc student
10. Nouran Elsayed, research assistant, and MSc student
11. Mohamed Shokry, research assistant and MSc student
Abdallah S. Abdelsattar, Warda M. Farouk, Shrouk Mohamed Gouda, Anan Safwat, Toka A. Hakim, Ayman El-Shibiny (2021). Utilization of Ocimum basilicum extracts for zinc oxide nanoparticles synthesis and their antibacterial activity after a novel combination with phage. Materials Letters. https://doi.org/10.1016/j.matlet.2021.131344.
Hamdy Abdelhamid, Omar E. Morsy, Ayman El-Shibiny, Reda Abdelbaset (2021). Detection of foodborne pathogens using novel vertical capacitive sensors. Alexandria Engineering Journal, https://doi.org/10.1016/j.aej.2021.09.016.
Mohamed S. Fayez, Toka A. Hakim, Mona M. Agwa, Mohamed Abdelmoteleb, Rania G. Aly, Nada N. Montaser, Abdallah S. Abdelsattar, Nouran Rezk and Ayman El-Shibiny (2021). Topically Applied Bacteriophage to Control Multi-Drug Resistant Klebsiella pneumoniae Infected Wound in a Rat Model. Antibiotics 2021, 10, 1048. https://doi.org/10.3390/antibiotics10091048.
Salsabil Makky, Alyaa Dawoud, Anan Safwat,Abdallah S.Abdelsattar, Nouran Rezk, Ayman El-Shibiny (2021). The Bacteriophage Decides Own tracks: When They are with or Against The Bacteria. Current Research in Microbial Sciences, 2 (2021) 100050. https://doi.org/10.1016/j.crmicr.2021.100050.
Abdallah S. Abdelsattar, Anan Safwat, Rana Nofal, Amera E. Taha, Salsabil Makky, Ayman El-Shibiny (2021). Isolation and Characterization of Bacteriophages ZCSE6 against Salmonella spp.: Phage Application in Milk. Biologics 1, 164–176. https://doi.org/10.3390/ biologics1020010.
Loay Bedda, Fayrouz Mahmoud, Radwa Klkhateib, Alyaa Dawoud, Hassan Gamal, Abdallah S. Abdelsattar, Ayman El-Shibiny (2021). SARS-CoV-2: Evaluation and Evolution. Coronaviruses. Volume 2, Issue 8, DOI: 10.2174/2666796702666210712104119.
Abdallah S. Abdelsattar, Alyaa Dawoud, Nouran Rezk, Salsabil Makky, Anan Safwat, Phil Richards and Ayman El Shibiny (2021). How to Train Your Phage: The Recent Efforts in Phage Training Biologics 2021, 1(2), 70-88; https://doi.org/10.3390/biologics1020005.
Shaimaa F. Mouftah, Jose´ F. Cobo-Dı´az, AvelinoA´ lvarez-Ordo´ ñez, Menattallah Elserafy, Nehal A. Saif1, Asmaa Sadat, Ayman El-Shibiny, Mohamed Elhadidy (2021). High-throughput sequencing reveals genetic determinants associated with antibiotic resistance in Campylobacter spp. from farm-to-fork. PLoS ONE, 2021, 16(6 June).
Abdallah S. Abdelsattar, Rana Nofal, Salsabil Makky, Anan Safwat, Amera Taha and Ayman El-Shibiny (2021). The Synergistic Effect of Biosynthesized Silver Nanoparticles and Phage ZCSE2 as a Novel Approach to Combat Multidrug-Resistant Salmonella enterica. Antibiotics 2021, 10, 678. https://doi.org/10.3390/antibiotics10060678.
Abdallah S. Abdelsattar, Alyaa Dawoud, Salsabil Makky, Rana Nofal, Ramy K. Aziz and Ayman El-Shibiny (2021). Bacteriophages: From Isolation to Application. Current Pharmaceutical Biotechnology, 2021, 22, 1-24. DOI: 10.2174/1389201022666210426092002.
Abdallah S. Abdelsattar, Rana Nofal, Salsabil Makky, Amera El-Sayed, Ayman El-Shibiny (2021). A Modified High-Throughput Screening Protocol to Isolate Bacteriophages from Environmental Samples. Preprints 2021, 2021020410 (doi: 10.20944/preprints202102.0410.v1)
Alsaadi, A., Beamud, B., Easwaran, M., Abdelrahman, F., Ayman El-Shibiny, Alghoribi, M.F., Domingo-Calap, P (2021).Learning From Mistakes: The Role of Phages in Pandemics. Frontiers in Microbiology, 2021, 12, 653107.
Mohamed El-Telbany, Gamal El-Didamony, Ahmed Askora, Eman Ariny, Dalia Abdallah, Ian F. Connerton, and Ayman El-Shibiny (2021). Bacteriophages to Control Multi-Drug Resistant Enterococcus faecalis Infection of Dental Root Canals. Microorganisms 2021, 9, 517. https://doi.org/ 10.3390/microorganisms9030517.
Fatma Abdelrahman, Maheswaran Easwaran , Oluwasegun I. Daramola, Samar Ragab, Stephanie Lynch, Tolulope J. Oduselu, Fazal Mehmood Khan, Akomolafe Ayobami, Fazal Adnan, Eduard Torrents, Swapnil Sanmukh, and Ayman El-Shibiny (2021) Phage-Encoded Endolysins. Antibiotics 2021, 10, 124. https://doi.org/10.3390/ antibiotics10020124.
El-Shibiny A., Dawoud A. (2020) Bacteriophage Applications for Food Safety. In: Witzany G. (eds) Biocommunication of Phages. Springer, Cham. https://doi.org/10.1007/978-3-030-45885-0_21. Print ISBN978-3-030-45884-3
Ahmed Mohamed, Omar Taha, Hesham M. El-Sherif, Phillippa L. Connerton, Steven P.T. Hooton, Nabil D. Bassim, Ian F. Connerton, and Ayman El-Shibiny (2020). Bacteriophage ZCSE2 is a potent antimicrobial against Salmonella enterica serovars: ultrastructure, genomics and efficacy. Accepted with revision. Viruses 2020, 12, 424; doi:10.3390/v12040424.
Mohamed Elhadidy, Mohamed Medhat Ali, Ayman El-Shibiny, William G. Miller, Walid F. Elkhatib, Nadine Botteldoorn, Katelijne Dierick (2020). Antimicrobial resistance patterns and molecular resistance markers of Campylobacter jejuni isolates from human diarrheal cases. https://doi.org/10.1371/journal.pone.0227833.
Hadeel Hosney, Eslam G. Al Sakkari, Ahmad Mustafa, Ibrahim Ashour, Ibrahim Mustafa, Ayman El Shibiny (2019). A cleaner enzymatic approach for producing non phthalate plasticiser to replace toxic based phthalates. Clean Technologies and Environmental Policy. https://doi.org/10.1007/s10098-019-01770-5.
Kamel S. Sultan, Tamer A. Ali, Nada A. Fahmy, Ayman El-Shibiny (2019). Using millimeter-waves for rapid detection of pathogenic bacteria in food based on bacteriophage. Engineering Reports. 2019;e12026. https://doi.org/10.1002/eng2.12026.
Abdallah S. Abdelsattar, Fatma Abdelrahman, Alyaa Dawoud, Ian F. Connerton and Ayman El-Shibiny (2019). Encapsulation of E. coli phage ZCEC5 in chitosan-alginate beads as a delivery system in phage therapy. AMB Express. 17;9(1):87. doi: 10.1186/s13568-019-0810-9.
Omar A. Taha, Phillippa L. Connerton, Ian F. Connerton and Ayman El-Shibiny (2018). Bacteriophage ZCKP1: A Potential Treatment for Klebsiella pneumoniae Isolated From Diabetic Foot Patients. Front. Microbiol. doi: 10.3389/fmicb.2018.02127.
Hadeel Hosney, Bassant Nadiem, Ibrahim Ashour, Ibrahim Mustafa, Ayman El-Shibiny (2018). Epoxidized vegetable oil and bio-based materials as PVC plasticizer. J. APPL. POLYM. SCI., DOI: 10.1002/APP.46270.
Ayman El-Shibiny and Salma El-Sahhar (2017) Bacteriophages: the possible solution to treat infections caused by pathogenic bacteria. Can J Microbiol. Nov; 63(11):865-879. doi: 10.1139/cjm-2017-0030.
Ayman El-Shibiny, Salma El-Sahhar, Mohamed Adel (2017) Phage applications for improving food safety and infection control in Egypt. Journal of Applied Microbiology. Journal of Applied Microbiology.123(2):556-567. DOI: 10.1111/jam.13500.
For the rest of the publications: